N-terminal cysteines essential for Golgi sorting of B-50 (GAP-43) in PC12 cells
نویسندگان
چکیده
منابع مشابه
Factors influencing GAP-43 gene expression in PC12 pheochromocytoma cells.
We have studied factors controlling message levels for the neuronal growth- and plasticity-associated protein, GAP-43. Following exposure of PC12 cells to various effectors, cytoplasmic RNA was isolated and analyzed by Northern transfer and autoradiography using a GAP-43 cDNA probe. Induction by NGF is apparent after 3 hr exposure and reaches maximal levels at 24 hr. Beyond 24 hr, levels remain...
متن کاملAnatomical distribution of the growth-associated protein GAP-43/B-50 in the adult rat brain.
GAP-43 (B-50,F1,pp46) is a neuron-specific phosphoprotein that has been implicated in the development and modulation of synaptic relationships. Although most neurons cease expressing high levels of GAP-43 after the completion of synaptogenesis (Jacobson et al., 1986), certain brain regions continue to have considerable amounts of the protein throughout life (Oestreicher et al., 1986); in at lea...
متن کاملLocalization of the growth-associated phosphoprotein GAP-43 (B-50, F1) in the human cerebral cortex.
The growth-associated phosphoprotein GAP-43 is a component of the presynaptic membrane that has been linked to the development and functional modulation of neuronal connections. A monospecific antibody raised against rat GAP-43 was used here to study the distribution of the protein in cortical and subcortical areas of the human brain. On Western blots, the antibody recognized a synaptosomal pla...
متن کاملThe prescriptions from Shenghui soup enhanced neurite growth and GAP-43 expression level in PC12 cells
BACKGROUND Shenghui soup is a traditional Chinese herbal medicine used in clinic for the treatment of forgetfulness. In order to understanding the prescription principle, the effects of "tonifying qi and strengthening spleen" group (TQSS) including Poria cocos (Schw.) Wolf. and Panax ginseng C.A.Mey and "eliminating phlegm and strengthening intelligence" group (EPSI) composed of Polygala tenuif...
متن کاملB-50/GAP-43-induced formation of filopodia depends on Rho-GTPase.
In the present study we show that expression of the neural PKC-substrate B-50 (growth-associated protein [GAP-43]) in Rat-1 fibroblasts induced the formation of filopodial extensions during spreading. This morphological change was accompanied by an enhanced formation of peripheral actin filaments and by accumulation of vinculin immunoreactivity in filopodial focal adhesions, colocalizing with B...
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ژورنال
عنوان ژورنال: NeuroReport
سال: 1995
ISSN: 0959-4965
DOI: 10.1097/00001756-199505090-00005